December 30, 1966Science
Nuclei from Rat Liver: Isolation Method That Combines Purity with High Yield
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Implication
Laboratory protocol demonstrates near-quantitative separation of clean cell nuclei in rat liver homogenates, indicating improved preservation for subsequent cytoplasmic fractionation.
Key Points
- Develop an improved isolation procedure that achieves high purity and quantitative yield of rat liver nuclei while preserving cytoplasmic components.
- Modified existing tissue isolation techniques by using concentrated sucrose solutions rather than citric acid or detergents.
- Processed whole rat liver homogenates to isolate nuclear fractions without destroying surrounding cellular material.
- Achieved near-quantitative separation of clean, intact nuclei from rat liver homogenates.
- Preserved cytoplasmic fractions in a state suitable for further quantitative separation and downstream analysis.
Cite This Study
Blobel et al. (1966) studied this question.