What question did this study set out to answer?

To develop a rapid, mild biochemical method for isolating intact nuclear membranes from rat liver nuclei while preserving structural integrity and enzymatic activity.

October 1, 1972European Journal of BiochemistryOpen Access

A Method for the Rapid Isolation of Nuclear Membranes from Rat Liver

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Authors

RKRobert R. KayMRC Laboratory of Molecular Biology DFDerek FraserUniversity College London IJI.R. JohnstonUniversity College London

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Implication

Biochemical study demonstrates intact nuclear membrane isolation using DNAase I in rat liver nuclei, suggesting an active role in DNA replication.

Key Points

To develop a rapid, mild biochemical method for isolating intact nuclear membranes from rat liver nuclei while preserving structural integrity and enzymatic activity.
Subjected isolated rat liver nuclei to double enzymatic digestion with low concentrations of DNAase I in ~0.1 mM Mg2+ at slightly alkaline pH.
Assessed membrane morphology via electron microscopy and quantified recovery of nuclear proteins, phospholipids, RNA, DNA, and marker enzyme activity.
Produced large double-membrane fragments with preserved nuclear pores and ribosomes, recovering approximately 55% of nuclear phospholipid, 8% of protein, 8% of RNA, and 1–3% of DNA.
Identified the nuclear membrane as the sole nuclear location of glucose-6-phosphatase and recovered approximately 1% of total nuclear DNA polymerase bound to the membrane.

Cite This Study

Kay et al. (1972) studied this question.

synapsesocial.com/papers/6a19d0963f3ec013f0df1989 https://doi.org/10.1111/j.1432-1033.1972.tb02081.x

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Implication

Biochemical study demonstrates intact nuclear membrane isolation using DNAase I in rat liver nuclei, suggesting an active role in DNA replication.

A Method for the Rapid Isolation of Nuclear Membranes from Rat Liver

Discussion

Member takes

Implication

Key Points

Cite This Study

A Method for the Rapid Isolation of Nuclear Membranes from Rat Liver

Discussion

Member takes

Implication

Key Points

Cite This Study

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