What question did this study set out to answer?

To establish and describe the 4T1 mouse mammary carcinoma model for orthotopic transplantation, surgical resection, and precise quantification of spontaneous metastatic spread.

October 1, 2000Current Protocols in Immunology

Mouse 4T1 Breast Tumor Model

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Authors

BPBeth A. PulaskiUniversity of Maryland, Baltimore County SOSuzanne Ostrand‐RosenbergUniversity of Utah

Discussion

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Implication

Preclinical protocol demonstrates spontaneous multi-organ metastasis in a murine breast cancer model, highlighting its utility for evaluating post-surgical metastatic disease.

Key Points

To establish and describe the 4T1 mouse mammary carcinoma model for orthotopic transplantation, surgical resection, and precise quantification of spontaneous metastatic spread.
Orthotopic transplantation of 4T1 carcinoma cells directly into the murine mammary gland fat pad.
Surgical excision of primary mammary tumors to model post-operative clinical metastatic recurrence.
Clonogenic assay using 6-thioguanine resistance to quantify disseminated micrometastatic tumor cells in distant tissues.
4T1 primary tumors spontaneously metastasize to draining lymph nodes, blood, liver, lungs, brain, and bone, mimicking advanced human disease.
Intrinsic resistance to 6-thioguanine enables sensitive detection and quantitation of isolated, sub-microscopic metastatic cells across distant anatomical sites.

Cite This Study

Pulaski et al. (2000) studied this question.

synapsesocial.com/papers/6a19da3a196cd56b09ead00c https://doi.org/10.1002/0471142735.im2002s39

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October 1, 2000Current Protocols in Immunology

Mouse 4T1 Breast Tumor Model

View Full Paper

Ask AI

Bookmark

Authors

BPBeth A. PulaskiUniversity of Maryland, Baltimore County SOSuzanne Ostrand‐RosenbergUniversity of Utah

Discussion

Member takes

Implication

Preclinical protocol demonstrates spontaneous multi-organ metastasis in a murine breast cancer model, highlighting its utility for evaluating post-surgical metastatic disease.

Key Points

To establish and describe the 4T1 mouse mammary carcinoma model for orthotopic transplantation, surgical resection, and precise quantification of spontaneous metastatic spread.
Orthotopic transplantation of 4T1 carcinoma cells directly into the murine mammary gland fat pad.
Surgical excision of primary mammary tumors to model post-operative clinical metastatic recurrence.
Clonogenic assay using 6-thioguanine resistance to quantify disseminated micrometastatic tumor cells in distant tissues.
4T1 primary tumors spontaneously metastasize to draining lymph nodes, blood, liver, lungs, brain, and bone, mimicking advanced human disease.
Intrinsic resistance to 6-thioguanine enables sensitive detection and quantitation of isolated, sub-microscopic metastatic cells across distant anatomical sites.

Cite This Study

Pulaski et al. (2000) studied this question.

synapsesocial.com/papers/6a19da3a196cd56b09ead00c https://doi.org/10.1002/0471142735.im2002s39

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