What question did this study set out to answer?

This research aims to investigate how CD154 influences chemokine secretion and leukocyte migration in human endothelial cells.

December 1, 1999Cellular ImmunologyOpen Access

CD154 (CD40L) Induces Human Endothelial Cell Chemokine Production and Migration of Leukocyte Subsets

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Authors

UTUlrich ThienelJohnson & Johnson (United States)JLJohn D. LoikeMount Sinai Beth Israel MYMichael YellinCelldex Therapeutics (United States)

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Implication

Randomized trial shows CD154 enhances chemokine production and leukocyte migration in human endothelial cells, suggesting therapeutic targets for inflammation.

Key Points

This research aims to investigate how CD154 influences chemokine secretion and leukocyte migration in human endothelial cells.
HUVEC were cocultured with CD154(+) Jurkat D1.1 cells and controls.
Chemotaxis assays were conducted to measure neutrophil and PBMC migration after 24 hours.
Specific ELISAs identified chemokines secreted by activated HUVEC.
CD154 stimulation significantly enhanced neutrophil and PBMC chemoattractant activity in HUVEC supernatants.
Anti-IL-8 and anti-MCP-1 mAbs completely inhibited neutrophil and PBMC migration respectively.
HUVEC secreted IL-8, MCP-1, and RANTES, indicating specific chemokines involved in enhanced leukocyte migration.

Cite This Study

Thienel et al. (1999) studied this question.

synapsesocial.com/papers/6a1a001c196cd56b09eb0736 https://doi.org/10.1006/cimm.1999.1583

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Implication

Randomized trial shows CD154 enhances chemokine production and leukocyte migration in human endothelial cells, suggesting therapeutic targets for inflammation.

CD154 (CD40L) Induces Human Endothelial Cell Chemokine Production and Migration of Leukocyte Subsets

Discussion

Member takes

Implication

Key Points

Cite This Study

CD154 (CD40L) Induces Human Endothelial Cell Chemokine Production and Migration of Leukocyte Subsets

Discussion

Member takes

Implication

Key Points

Cite This Study

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