Iridium–Schiff base complex-based fluorimetric turn-off sensor and HPLC-DAD analytical approaches for quantitation of 8-hydroxy-2′-deoxyguanosine as an oxidative DNA damage biomarker in biofluids of healthy and diabetic subjects

Hazim M. Ali; Tamer H. A. Hasanin; Ahmed Hamad Alanazi; Emad Manni; Moamen S. Refat; Azza H. Rageh; Mohammed Gamal

doi:10.1039/d6ra02144a

January 1, 2026RSC AdvancesOpen Access

Iridium–Schiff base complex-based fluorimetric turn-off sensor and HPLC-DAD analytical approaches for quantitation of 8-hydroxy-2′-deoxyguanosine as an oxidative DNA damage biomarker in biofluids of healthy and diabetic subjects

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Key Points

This research aims to develop a sensor for detecting 8-hydroxy-2′-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage, in biofluids.
Utilized an iridium-Schiff base complex for fluorimetric measurements.
Employed high-performance liquid chromatography with diode array detection (HPLC-DAD) for analysis.
Assessed samples from healthy individuals and diabetic subjects.
8-OHdG effectively quenched fluorescence of the Ir-SB complex across a broad linear range.
Both spectrofluorimetry and chromatography successfully detected 8-OHdG in various biofluids.
Higher levels of 8-OHdG were observed in biofluids of diabetic subjects compared to healthy volunteers.

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Implication

Quantifies oxidative DNA damage biomarker 8-OHdG in biofluids, indicating health differences between groups.

Abstract

8-OHdG selectively quenches Ir-SB complex fluorescence over a broad linear range. Native spectrofluorimetry and chromatographic methods were applied for the determination of 8-OHdG in biofluids from healthy volunteers and diabetic patients.

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Ali et al. (2026) studied this question.

synapsesocial.com/papers/6a1a80550307b7850943257e https://doi.org/10.1039/d6ra02144a

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Iridium–Schiff base complex-based fluorimetric turn-off sensor and HPLC-DAD analytical approaches for quantitation of 8-hydroxy-2′-deoxyguanosine as an oxidative DNA damage biomarker in biofluids of healthy and diabetic subjects

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Abstract

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Cite This Study

Iridium–Schiff base complex-based fluorimetric turn-off sensor and HPLC-DAD analytical approaches for quantitation of 8-hydroxy-2′-deoxyguanosine as an oxidative DNA damage biomarker in biofluids of healthy and diabetic subjects

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Abstract

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