What question did this study set out to answer?

Determine the fractional degradation rates and overall ruminal degradability of specific corn protein subfractions using gel electrophoresis and densitometry.

April 1, 1994Journal of Dairy ScienceOpen Access

Electrophoretic Analysis of Ruminal Degradability of Corn Proteins

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Authors

DRDonato F. RomagnoloVita-Salute San Raffaele University CPC.E. PolanVirginia Tech WBWilliam E. BarbeauVirginia Tech

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Implication

In situ digestion study reveals differential ruminal degradability across corn protein fractions in lactating dairy cows, highlighting the high ruminal resistance of zein prolamins.

Key Points

Determine the fractional degradation rates and overall ruminal degradability of specific corn protein subfractions using gel electrophoresis and densitometry.
Extracted albumin, globulin, and prolamin fractions from corn meal and corn gluten meal using selective solvent extraction and characterized them via SDS-PAGE and densitometric scanning.
Suspended corn meal and corn gluten meal samples in situ in the rumen of lactating dairy cows across a 0 to 72 hour incubation timeline to evaluate residual protein degradation.
Zein prolamins were significantly more resistant to ruminal degradation than albumins, globulins, and glutelins, with fractional degradation rates of 0.026/h versus 0.060/h in corn and 0.015/h versus 0.018/h in corn gluten meal.
Total ruminal degradability determined by summing the disappearance of individual subfractions was 52.2% for corn meal and 18.6% for corn gluten meal.

Cite This Study

Romagnolo et al. (1994) studied this question.

synapsesocial.com/papers/6a1bc4fab33628da419cd433 https://doi.org/10.3168/jds.s0022-0302(94)77044-2

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Implication

In situ digestion study reveals differential ruminal degradability across corn protein fractions in lactating dairy cows, highlighting the high ruminal resistance of zein prolamins.

Electrophoretic Analysis of Ruminal Degradability of Corn Proteins

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Implication

Key Points

Cite This Study

Electrophoretic Analysis of Ruminal Degradability of Corn Proteins

Discussion

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Implication

Key Points

Cite This Study

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