What does this research mean for the field?

Adrenaline induces a slight increase in the phosphorylation of the P light chain of myosin and troponin I in the beating heart, with maximum phosphorylation occurring after the peak inotropic response. Novelty: ClaimNovelty.CONFIRMATORY. Consensus alignment: ConsensusAlignment.SUPPORTS_CONSENSUS.

What question did this study set out to answer?

To determine the extent and timing of myosin P light chain and troponin I phosphorylation in perfused beating rabbit hearts exposed to adrenaline or ischemia.

July 1, 1981Biochemical JournalOpen Access

The effect of adrenaline on the phosphorylation of the P light chain of myosin and troponin I in the perfused rabbit heart

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Why the study?

Does adrenaline alter the phosphorylation of the P light chain of myosin and troponin I in the perfused rabbit heart?

Population

Perfused rabbit beating heart (and ventricles of rabbit, guinea pig, and cow)

Comparison

Adrenaline vs Baseline/absence of adrenaline

Design

Preclinical

Authors

SWSara A. WestwoodHome Office SPS V PerryUniversity of California, Los Angeles

Discussion

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Implication

Adrenaline's delayed phosphorylation effects in rabbit hearts; leaves open relevance to human inotropic responses.

Key Points

To determine the extent and timing of myosin P light chain and troponin I phosphorylation in perfused beating rabbit hearts exposed to adrenaline or ischemia.
Utilized two-dimensional electrophoresis combined with a sample preparation technique designed to eliminate endogenous protein phosphatase activity.
Assessed phosphorylated and unphosphorylated protein fractions in perfused beating rabbit hearts and ventricular tissue from rabbits, guinea pigs, and cows.
Approximately 25% of the ventricular myosin P light-chain fraction was phosphorylated under baseline conditions both in vivo and in perfused beating hearts.
Adrenaline administration induced a slight increase in the phosphorylation of both myosin P light chain and troponin I, peaking after the maximum inotropic response.
Myocardial ischemia in the absence of adrenaline produced an increase in P light chain phosphorylation comparable to that seen with adrenaline.

Structured PICO

Does adrenaline alter the phosphorylation of the P light chain of myosin and troponin I in the perfused rabbit heart?

Population

Perfused rabbit beating heart (and ventricles of rabbit, guinea pig, and cow)

Intervention

Adrenaline

Comparator

Baseline/absence of adrenaline

Outcome

Extent of phosphorylation of the P light chain of myosin and troponin Isurrogate

Adrenaline induces a slight, delayed increase in the phosphorylation of the P light chain of myosin and troponin I in the perfused rabbit heart, peaking after the maximal inotropic response.

Cite This Study

Westwood et al. (1981) studied this question.

synapsesocial.com/papers/6a1bcd6e0a1f7575939cf2cd https://doi.org/10.1042/bj1970185

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The effect of adrenaline on the phosphorylation of the P light chain of myosin and troponin I in the perfused rabbit heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

The effect of adrenaline on the phosphorylation of the P light chain of myosin and troponin I in the perfused rabbit heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider