Key result
Changes in cardiac contractile state induced by various perfusions in rabbit and rat hearts were not accompanied by changes in the extent of phosphorylation of P-light chain.
Population
Working perfused rabbit and rat hearts
Comparison
Perfusion with 0.1 microM isoproterenol… vs Control perfused rabbit and rat hearts
Design
Preclinical
Follow-up
up to 30 minutes
Authors
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May link myosin phosphorylation to contractility in isolated hearts; leaves open translation to human physiology or therapy.
In perfused rabbit and rat hearts, alterations in cardiac contractility occur independently of changes in myosin P-light chain phosphorylation.
High et al. (1980) studied this question. Various perfusions (isoproterenol, increased calcium, calcium-free buffer, high potassium) vs. Control perfused hearts was evaluated on Myosin P-light chain phosphate content and LV dP/dtmax. Changes in cardiac contractile state induced by various perfusions in rabbit and rat hearts were not accompanied by changes in the extent of phosphorylation of P-light chain.
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