What question did this study set out to answer?

This research aims to explore how phosphorylation of contractile proteins affects cardiac function and responsiveness to catecholamines.

July 5, 1983Philosophical transactions of the Royal Society of London. Series B, Biological sciences

Cardiac function and phosphorylation of contractile proteins

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Population

Perfused hearts (including rat heart), in vitro preparations, and skinned fibre preparations

Comparison

Catecholamines, cholinergic agents, positive… vs Control conditions

Design

Preclinical

Authors

PEP. EnglandRutgers, The State University of New Jersey

Discussion

Member takes

Implication

May inform lusitropic effects of catecholamines in animals; leaves open translation to human diastolic function or therapies.

Key Points

This research aims to explore how phosphorylation of contractile proteins affects cardiac function and responsiveness to catecholamines.
Examined phosphorylation levels of troponin-I and C-protein in perfused hearts in response to catecholamines.
Measured effects of cyclic-AMP-dependent protein kinase on contractile proteins in vitro.
Analyzed the dephosphorylation rates of these proteins in the presence of cholinergic agents.
Phosphorylation of troponin-I decreases sensitivity to calcium and accelerates relaxation rates with catecholamine stimulation.
C-protein phosphorylation reaches 4-5 mol phosphate/mole protein; its functions remain unclear.
Dephosphorylation of both proteins is slower compared to phosphorylase, suggesting different regulatory mechanisms.

Structured PICO

Population

Perfused hearts (including rat heart), in vitro preparations, and skinned fibre preparations

Intervention

Catecholamines, cholinergic agents, positive inotropic agents, and short-term ischaemia

Comparator

Control conditions

Outcome

Phosphorylation and dephosphorylation of contractile proteins (Troponin-I, C-protein, myosin P-light chain) and their effects on Ca2+ sensitivity and tension developmentsurrogate

Phosphorylation of troponin-I plays a key role in the increased rate of cardiac relaxation observed with catecholamine stimulation by decreasing calcium sensitivity.

Cite This Study

P. England (1983) studied this question.

synapsesocial.com/papers/6a208eecb15f64b498301f5f https://doi.org/10.1098/rstb.1983.0040

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Cardiac function and phosphorylation of contractile proteins

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Cardiac function and phosphorylation of contractile proteins

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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