What does this research mean for the field?

Tumor necrosis factor-alpha (TNF-alpha) pretreatment protects adult mammalian cardiac myocytes against hypoxic injury through a mechanism that is independent of heat shock protein 72 expression and involves activation of both type 1 and 2 TNF receptors. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This research aims to determine if TNF-alpha can protect cardiac myocytes from hypoxic injury.

April 14, 1998Circulation

Tumor Necrosis Factor-α Confers Resistance to Hypoxic Injury in the Adult Mammalian Cardiac Myocyte

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Why the study?

Does TNF-alpha pretreatment prevent hypoxic injury in isolated adult mammalian cardiac myocytes?

Population

Isolated adult mammalian cardiac myocytes

Comparison

Pretreatment with tumor necrosis factor-alpha ≥… vs Diluent-treated cells subjected to the same…

Design

Preclinical

Follow-up

12 hours of hypoxia followed by reoxygenation

Authors

MNMasayuki NakanoRIKEN Center for Biosystems Dynamics Research AKAnne A. KnowltonHeart Failure / Cardiomyopathy ZDZiad DibbsMerck & Co., Inc., Rahway, NJ, USA (United States)

Discussion

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Implication

Hypothesis-generating for TNF-alpha in myocyte protection; leaves open clinical translation from this animal model.

Key Points

This research aims to determine if TNF-alpha can protect cardiac myocytes from hypoxic injury.
Isolated adult cardiac myocytes were pretreated with TNF-alpha for 12 hours.
Cells were exposed to hypoxic conditions (O2 content, 3 to 5 ppm) for 12 hours, followed by reoxygenation.
Cell injury was assessed using LDH release, calcium uptake, and MTT metabolism.
TNF-alpha pretreatment (≥ 50 U/mL) significantly reduced LDH release during hypoxia compared to controls.
Similar reductions were observed in 45Ca2+ uptake and MTT metabolism in TNF-alpha pretreated cells.
Activation of both TNF receptors was necessary for the protective effect, indicating a novel protective mechanism.

Structured PICO

Does TNF-alpha pretreatment prevent hypoxic injury in isolated adult mammalian cardiac myocytes?

Population

Isolated adult mammalian cardiac myocytes

Intervention

Pretreatment with tumor necrosis factor-alpha (TNF-alpha) ≥ 50 U/mL for 12 hours

Comparator

Diluent-treated cells subjected to the same hypoxic injury

Outcome

Cell injury assessed by lactic dehydrogenase (LDH) release, 45Ca2+ uptake, and MTT metabolism after 12 hours of continuous hypoxic injury followed by reoxygenationsurrogate

TNF-alpha pretreatment protects adult cardiac myocytes from hypoxic injury via a mechanism independent of HSP 72 expression, involving both type 1 and 2 TNF receptors.

Cite This Study

Nakano et al. (1998) studied this question.

synapsesocial.com/papers/6a1bd4451567d2fc4d5f1871 https://doi.org/10.1161/01.cir.97.14.1392

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Tumor Necrosis Factor-α Confers Resistance to Hypoxic Injury in the Adult Mammalian Cardiac Myocyte

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Tumor Necrosis Factor-α Confers Resistance to Hypoxic Injury in the Adult Mammalian Cardiac Myocyte

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

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