What does this research mean for the field?

A specific 119-base pair sequence in the 5'-flanking region of the endothelin-1 gene, which includes an AP1 consensus sequence, functions as an endothelial cell-specific cis-acting element that regulates its transcription. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

June 1, 1990Journal of Biological ChemistryOpen Access

Functional analysis of the endothelin-1 gene promoter. Evidence for an endothelial cell-specific cis-acting sequence.

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Population

Bovine aortic endothelial cells

Comparison

Transfection with fusion plasmids containing… vs Constructs with different lengths of ET-1…

Design

Preclinical

Authors

MLM E LeeAerospace Center Hospital KBKenneth D. BlochHarvard University JCJane CliffordDrexel University

Discussion

Member takes

Implication

Maps ET-1 promoter elements for endothelial specificity; leaves open in vivo validation or therapeutic targeting.

Structured PICO

Population

Bovine aortic endothelial cells

Intervention

Transfection with fusion plasmids containing ET-1 5'-flanking sequence and the chloramphenicol acetyltransferase gene

Comparator

Constructs with different lengths of ET-1 5'-flanking sequence (e.g., 129 base pairs vs 143 base pairs) or heterologous promoter

Outcome

Rate of transcription of the ET-1 genesurrogate

Identifies specific cis-acting sequences in the ET-1 gene promoter that direct its transcription rate and endothelial cell specificity.

Cite This Study

Lee et al. (1990) studied this question.

synapsesocial.com/papers/6a1bf0bcd54006be995f43ce https://doi.org/10.1016/s0021-9258(18)86967-8

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Functional analysis of the endothelin-1 gene promoter. Evidence for an endothelial cell-specific cis-acting sequence.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Functional analysis of the endothelin-1 gene promoter. Evidence for an endothelial cell-specific cis-acting sequence.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider