What question did this study set out to answer?

This research aims to evaluate how reducing disulphide bridges influences protein mobility in polyacrylamide gels.

February 1, 1972Biochemical JournalOpen Access

The effect of cross-links on the mobility of proteins in dodecyl sulphate–polyacrylamide gels

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Authors

IGIan P. GriffithUniversity of London

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Implication

Randomized trial examines how disulphide bridge reduction affects protein mobility in gel electrophoresis, suggesting important considerations for molecular weight estimates.

Key Points

This research aims to evaluate how reducing disulphide bridges influences protein mobility in polyacrylamide gels.
Prepared polypeptide polymers with varying cystine bridges using glutaraldehyde.
Denatured proteins with heat, sodium dodecyl sulphate, and urea, then analyzed gel mobility post-reduction with dithiothreitol.
Compared mobilities of polypeptides with and without cystine before and after reduction.
Reduction decreased mobilities of cystine-containing polypeptides, varying by original cystine content and protein source.
Mobilities of lysozyme monomers and ribonuclease dimers increased after reduction.
Reduced polypeptide oligomers migrated faster than expected based on molecular weights.

Cite This Study

Ian P. Griffith (1972) studied this question.

synapsesocial.com/papers/6a1bfb284ebd09f3dfa94a6e https://doi.org/10.1042/bj1260553

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Implication

Randomized trial examines how disulphide bridge reduction affects protein mobility in gel electrophoresis, suggesting important considerations for molecular weight estimates.

The effect of cross-links on the mobility of proteins in dodecyl sulphate–polyacrylamide gels

Discussion

Member takes

Implication

Key Points

Cite This Study

The effect of cross-links on the mobility of proteins in dodecyl sulphate–polyacrylamide gels

Discussion

Member takes

Implication

Key Points

Cite This Study

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