What is the clinical evidence from this study?

Study design: Other. Population: Neonatal rat cardiac myocytes (in vitro). Intervention: Phorbol ester (4beta-PMA). Primary outcome: In vitro phosphorylation of COIV and cytochrome c oxidase activity.

What does this research mean for the field?

In neonatal rat cardiac myocytes, protein kinase C epsilon (PKCepsilon) interacts with cytochrome c oxidase subunit IV (COIV) in mitochondria, leading to a 2-fold enhancement of electron-transport chain complex IV activity. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

September 7, 2004Biochemical JournalOpen Access

Cytochrome c oxidase subunit IV as a marker of protein kinase Cε function in neonatal cardiac myocytes: implications for cytochrome c oxidase activity

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Key result

In neonatal rat cardiac myocytes, PKCepsilon interacts with cytochrome c oxidase subunit IV (COIV) in mitochondria, enhancing electron-transport chain complex IV activity 2-fold.

Population

Neonatal rat cardiac myocytes (NCMs)

Comparison

Phorbol ester treatment and PKCepsilon-selective… vs Untreated cells

Design

Preclinical

Authors

MOMourad OgbiAugusta University CCCatherine S. ChewUppsala University JPJan PohlCenters for Disease Control and Prevention

Discussion

Member takes

Implication

Hypothesis-generating in neonatal rat myocytes; leaves open PKCepsilon's role in human cardiac energetics.

Structured PICO

Population

Neonatal rat cardiac myocytes (NCMs)

Intervention

Phorbol ester treatment (4beta-PMA) and PKCepsilon-selective translocation inhibitor

Comparator

Untreated cells

Outcome

In vitro phosphorylation of cytochrome c oxidase subunit IV (COIV) and cytochrome c oxidase activitysurrogate

PKCepsilon interacts with COIV in neonatal cardiac myocyte mitochondria to enhance electron-transport chain complex IV activity, providing a novel approach for monitoring PKCepsilon function.

Cite This Study

Ogbi et al. (2004) studied Neonatal rat cardiac myocytes (in vitro). Phorbol ester (4beta-PMA) was evaluated on In vitro phosphorylation of COIV and cytochrome c oxidase activity. In neonatal rat cardiac myocytes, PKCepsilon interacts with cytochrome c oxidase subunit IV (COIV) in mitochondria, enhancing electron-transport chain complex IV activity 2-fold.

synapsesocial.com/papers/6a1c2f1b1567d2fc4d5fb9fa https://doi.org/10.1042/bj20040468

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Cytochrome c oxidase subunit IV as a marker of protein kinase Cε function in neonatal cardiac myocytes: implications for cytochrome c oxidase activity

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Cytochrome c oxidase subunit IV as a marker of protein kinase Cε function in neonatal cardiac myocytes: implications for cytochrome c oxidase activity

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider