What does this research mean for the field?

In rat ventricular myocytes, cellular Ca2+ flux balance across varying stimulation frequencies is maintained by a combination of increased efflux driven by elevated diastolic [Ca2+]i and decreased influx via L-type Ca2+ current. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

October 12, 2007The Journal of PhysiologyOpen Access

Regulation of systolic [Ca²⁺]i and cellular Ca²⁺ flux balance in rat ventricular myocytes by SR Ca²⁺, L‐type Ca²⁺ current and diastolic [Ca²⁺]i

Q: What is the clinical evidence from this study?

Population: Normal physiology (force-frequency response). Intervention: Stimulation frequency and beta-adrenergic stimulation (isoprenaline). Primary outcome: Systolic Ca2+ transient, SR Ca2+ content, L-type Ca2+ current, and diastolic [Ca2+]i.

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Key result

In rat ventricular myocytes, Ca2+ flux balance across stimulation frequencies is maintained by increased efflux due to elevated diastolic [Ca2+]i (contributing 69.3 +/- 6% at 8 Hz) and decreased influx.

Population

Single rat ventricular myocytes

Comparison

Alteration of stimulation frequency and… vs Baseline or different stimulation frequencies…

Design

Preclinical

Authors

KDKatharine M. DibbUniversity of Manchester DEDavid EisnerElectrophysiology ATAndrew W. TraffordElectrophysiology

Discussion

Member takes

Implication

Advances rodent myocyte Ca2+ homeostasis models; hypothesis-generating for human frequency-dependent regulation.

Structured PICO

Population

Single rat ventricular myocytes

Intervention

Alteration of stimulation frequency (1-8 Hz) and beta-adrenergic stimulation (isoprenaline, 30 nM) under current clamp and voltage clamp conditions

Comparator

Baseline or different stimulation frequencies without beta-adrenergic stimulation

Outcome

Systolic Ca2+ transient amplitude, sarcoplasmic reticulum (SR) Ca2+ content, L-type Ca2+ current (ICa-L), and diastolic [Ca2+]isurrogate

In rat ventricular myocytes, cellular Ca2+ flux balance during varying stimulation frequencies is maintained by increased efflux from elevated diastolic Ca2+ and decreased influx via L-type Ca2+ channels.

Cite This Study

Dibb et al. (2007) studied Normal physiology (force-frequency response). Stimulation frequency and beta-adrenergic stimulation (isoprenaline) was evaluated on Systolic Ca2+ transient, SR Ca2+ content, L-type Ca2+ current, and diastolic [Ca2+]i. In rat ventricular myocytes, Ca2+ flux balance across stimulation frequencies is maintained by increased efflux due to elevated diastolic [Ca2+]i (contributing 69.3 +/- 6% at 8 Hz) and decreased influx.

synapsesocial.com/papers/6a1c39cc4cc49ccc94a9186c https://doi.org/10.1113/jphysiol.2007.141473

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