What question did this study set out to answer?

To determine whether macrophages can independently oxidatively modify low-density lipoprotein (LDL) into a form recognized and degraded by the acetyl LDL scavenger receptor.

September 1, 1986Arteriosclerosis An Official Journal of the American Heart Association IncOpen Access

Macrophage oxidation of low density lipoprotein generates a modified form recognized by the scavenger receptor.

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To determine whether macrophages can independently oxidatively modify low-density lipoprotein (LDL) into a form recognized and degraded by the acetyl LDL scavenger receptor.
Incubated 125I-labeled LDL with resident or thioglycollate-elicited macrophages for 24 hours in Ham's F-10 medium or DMEM, with or without the antioxidant butylated hydroxytoluene (BHT).
Assessed LDL oxidation via thiobarbituric acid (TBA) reactive substances, electrophoretic mobility, and degradation assays in fresh macrophages using unlabeled acetyl LDL, endothelial-modified LDL, and native LDL as competitive inhibitors.
Incubation with macrophages generated TBA-reactive materials and markedly increased LDL electrophoretic mobility and subsequent degradation by fresh macrophages relative to cell-free controls.
Oxidative modification was prevented when the initial incubation included the antioxidant BHT or was conducted in DMEM instead of Ham's F-10 medium.
Degradation of macrophage-modified LDL was competitively inhibited by unlabeled acetyl LDL and endothelial cell-modified LDL, but not native LDL, confirming mediation by the acetyl LDL receptor.

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Implication

In vitro experiment demonstrates that macrophages oxidatively modify low-density lipoprotein, indicating that immune cells directly produce atherogenic ligands recognized by scavenger receptors.

Cite This Study

Parthasarathy et al. (1986) studied this question.

synapsesocial.com/papers/6a1c91caf63f086470a187eb https://doi.org/10.1161/01.atv.6.5.505

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Implication

In vitro experiment demonstrates that macrophages oxidatively modify low-density lipoprotein, indicating that immune cells directly produce atherogenic ligands recognized by scavenger receptors.

Macrophage oxidation of low density lipoprotein generates a modified form recognized by the scavenger receptor.

Key Points

Discussion

Implication

Cite This Study

Macrophage oxidation of low density lipoprotein generates a modified form recognized by the scavenger receptor.

Key Points

Discussion

Implication

Cite This Study

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