Non-Muscle Myosin II in Axonal Cell Biology: From the Growth Cone to the Axon Initial Segment

Figures

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  Figure 1. Non-muscle myosin II (NMII) structure and regulation. (A) NMII contains two heavy chains (HC), two regulatory light chains (RLCs) (orange) and two essential light chains (ELCs) (yellow). The heavy chain includes the head domain (green), with an actin binding site and an ATPase motor domain, the neck domain to which the ELC and RLC are bound, and the tail domain, with a helical coiled coil rod filament and a non-helical tail. In the absence of RLC phosphorylation, NMII is in an inactive conformation (left). Upon RLC phosphorylation by MLCK or ROCK, for instance, NMII unfolds to generate an active conformation (middle). MLCK phosphorylates RLC on Ser19 and Thr18, depicted as two red circles. The RLC phosphatase MLCP can revert this activation. NMII is then able to assemble into bipolar filaments, which bind to actin (blue) (right). (B) Upon NMII activation, polymerization-competent NMII molecules can form bipolar filaments through electrostatic interactions of their rod domains. Addition of more NMII molecules drives the growth of a bipolar NMII filament. For a matter of simplicity, a smaller number of myosins than that generally found on each side of the filament is drawn. (C)…

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  Figure 2. NMII in axon elongation, retraction and regeneration. (A) Schematic representation of the growth cone. The growth cone includes a central domain, mainly consisting of bundled microtubules (grey lines), a transition zone, enriched in actin arcs (blue hemicircular lines) and a peripheral domain, enriched in actin (blue lines) in the form of lamellipodia and filopodia. Actin bundles assemble near the growth cone leading edge, translocate rearward by retrograde flow towards the transition zone (blue dashed arrows in the peripheral domain) and recycle through bundle severing. NMIIA (green) is mainly located within the central domain and NMIIB (red) in the transition zone and peripheral domain. (B) General effect of NMII in neurite extension, axon elongation, retraction and regeneration. During early neurite extension pharmacological inhibition of NMII either directly using blebbistatin, or indirectly through the inhibition of the upstream regulators MLCK or ROCK, promotes axon elongation (upper left panel). When analyzing the effect of different NMII isoforms in axon elongation, in general terms, NMIIB is required for axon elongation (middle left panel) while NMIIA participates in…

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  Figure 3. Schematic representation of the role of the actomyosin cytoskeleton in the control of axonal contractility. Phosphorylated myosin light chain is highly enriched at the AIS, where it may participate in the regulation of axon diameter during action potential firing. In the axon shaft, circumferential and longitudinal axon tension are also regulated by the actomyosin network. While pMLC forms circular periodic structures colocalizing with membrane periodic skeleton (MPS) actin rings, NMII heavy chains appear mostly distributed as multiple filaments with approximately 300 nm of length along the longitudinal axonal axis, crosslinking adjacent actin rings (probably providing for tension); NMII filaments spanning individual rings may also exist (and provide for contraction).