What question did this study set out to answer?

To evaluate the metabolic consequences of glycerol and dihydroxyacetone loading on hepatic metabolite profiles and adenine nucleotide concentrations.

January 15, 1973Biochemical JournalOpen Access

The effect of glycerol and dihydroxyacetone on hepatic adenine nucleotides

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Authors

HWH. F. WoodsUniversity of Indianapolis HKH. A. KrebsDeutsche Forschungsgemeinschaft

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Implication

Laboratory study demonstrates glycerol depletes hepatic adenine nucleotides while dihydroxyacetone preserves them in rat livers, indicating distinct energetic costs during substrate metabolism.

Key Points

To evaluate the metabolic consequences of glycerol and dihydroxyacetone loading on hepatic metabolite profiles and adenine nucleotide concentrations.
Perfused isolated livers from fed and starved rats with media containing either glycerol or dihydroxyacetone.
Quantified substrate uptake and the release of glucose, lactate, and pyruvate into the perfusion medium.
Measured tissue concentrations of adenine nucleotides, alpha-glycerophosphate, fructose 1-phosphate, and inorganic phosphate, including trials in phosphate-free medium.
Glycerol loading caused alpha-glycerophosphate to surge from 0.13 to 8.45 µmol/g at 40 minutes and reduced tissue adenine nucleotides to 70% of control values.
Tissue inorganic phosphate fell from 4.25 to 2.31 µmol/g at 10 minutes following glycerol exposure, while omitting phosphate from the medium exacerbated adenine nucleotide depletion.
Dihydroxyacetone was cleared faster than glycerol without significantly diminishing adenine nucleotides, elevating fructose 1-phosphate from 0.23 to 0.39 µmol/g while keeping lactate-to-pyruvate ratios below 10.

Cite This Study

Woods et al. (1973) studied this question.

synapsesocial.com/papers/6a1cc86dc9d372840a89b52a https://doi.org/10.1042/bj1320055

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Implication

Laboratory study demonstrates glycerol depletes hepatic adenine nucleotides while dihydroxyacetone preserves them in rat livers, indicating distinct energetic costs during substrate metabolism.

The effect of glycerol and dihydroxyacetone on hepatic adenine nucleotides

Discussion

Member takes

Implication

Key Points

Cite This Study

The effect of glycerol and dihydroxyacetone on hepatic adenine nucleotides

Discussion

Member takes

Implication

Key Points

Cite This Study

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