What question did this study set out to answer?

To identify the structural and genetic determinants within HIV-1 envelope gp120 that enable dualtropic viruses to use CXCR4 for macrophage entry and evaluate their clinical significance.

October 14, 2005Journal of VirologyOpen Access

Complex Determinants in Human Immunodeficiency Virus Type 1 Envelope gp120 Mediate CXCR4-Dependent Infection of Macrophages

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Authors

GGGuity GhaffariQuest Diagnostics (United Kingdom)DTDaniel L. TuttleUniversity of Florida DBDaniel R. BriggsNorthwestern Memorial Hospital

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Implication

Virological study reveals complex gp120 mutations mediate CXCR4-dependent macrophage infection in HIV-1 quasispecies, indicating novel targets to prevent viral evolution.

Key Points

To identify the structural and genetic determinants within HIV-1 envelope gp120 that enable dualtropic viruses to use CXCR4 for macrophage entry and evaluate their clinical significance.
Analyzed viral quasispecies phenotypes and pathogenesis in peripheral blood and postmortem thymic tissue from infected individuals.
Mapped genetic determinants across gp120 domains mediating ex vivo macrophage infection via CXCR4.
Dualtropic D-X4 viral variants were significantly associated with advanced CD4+ T-cell attrition and co-occurred alongside M-R5 and T-X4 viruses in tissue and blood.
Infection of macrophages via CXCR4 required discontinuous amino acid changes across the V1/V2 domains, V3 loop, and V5 hypervariable region.
Coreceptor use and entry efficiency were driven by gp120 conformational adaptations rather than differences in total virion-associated gp120 levels.

Cite This Study

Ghaffari et al. (2005) studied this question.

synapsesocial.com/papers/6a1d1f5eba3016ff712f1a1f https://doi.org/10.1128/jvi.79.21.13250-13261.2005

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Implication

Virological study reveals complex gp120 mutations mediate CXCR4-dependent macrophage infection in HIV-1 quasispecies, indicating novel targets to prevent viral evolution.

Complex Determinants in Human Immunodeficiency Virus Type 1 Envelope gp120 Mediate CXCR4-Dependent Infection of Macrophages

Discussion

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Implication

Key Points

Cite This Study

Complex Determinants in Human Immunodeficiency Virus Type 1 Envelope gp120 Mediate CXCR4-Dependent Infection of Macrophages

Discussion

Member takes

Implication

Key Points

Cite This Study

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