An atomic-level lens on biomolecular condensation by NMR spectroscopy

Q: What does this research mean for the field?

Nuclear magnetic resonance (NMR) spectroscopy provides critical atomic- and residue-level insights into the interaction networks driving biomolecular phase separation and links liquid condensates to pathological solid aggregates. Novelty: ClaimNovelty.SYNTHESIS. Consensus alignment: ConsensusAlignment.NEUTRAL.

Philip O’Toole; Eurico J. Cabrita

doi:10.1016/j.molliq.2026.129709

May 1, 2026Journal of Molecular LiquidsOpen Access

An atomic-level lens on biomolecular condensation by NMR spectroscopy

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Key Points

The study aims to explore how NMR spectroscopy can provide detailed insights into biomolecular phase separation and condensation.
Applied chemical shift perturbation (CSP), relaxation, paramagnetic relaxation enhancement (PRE), and nuclear Overhauser effect (NOE) to map interaction networks.
Utilized solvent PRE, fluorine NMR, and systems NMR to enhance characterization of biomolecular condensates.
Implemented solid-state NMR to connect liquid condensates with pathological solid aggregates linked to disease.
NMR successfully mapped interaction networks in intrinsically disordered proteins (IDPs) undergoing phase separation.
Characterization methods using solvent PRE and 19F NMR improved understanding of biomolecular condensates.
Findings indicate a link between liquid condensates and solid aggregates, with implications for understanding disease mechanisms.

Implication

Examines interactions driving biomolecular phase separation, suggesting new characterization methods.

Abstract

• NMR provides residue-level insight into interactions driving biomolecular phase separation. • CSP, relaxation, PRE, NOE map interaction networks in phase-separating IDPs. • Solvent PRE, 19 F NMR, and systems NMR expand condensate characterization. • Solid-state NMR links liquid condensates to pathological solid aggregates in disease. • NMR integration with biophysical methods enables multiscale condensate analysis.

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Cite This Study

O’Toole et al. (2026) studied this question.

synapsesocial.com/papers/6a1d22db02fbce9130638797 https://doi.org/10.1016/j.molliq.2026.129709

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An atomic-level lens on biomolecular condensation by NMR spectroscopy

Key Points

Implication

Abstract

Discussion

Cite This Study

An atomic-level lens on biomolecular condensation by NMR spectroscopy

Key Points

Implication

Abstract

Discussion

Cite This Study

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