The study demonstrates that VPg-pUpU is synthesized in a membranous environment, suggesting it is part of the initiation complex in poliovirus RNA replication.
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Supports membranous VPg-pUpU synthesis in poliovirus replication; leaves open in vivo roles and therapeutic potential.
A small nucleotidyl-protein has been synthesized in vitro in a membrane fraction of poliovirus-infected HeLa cells. Analyses of the nucleotides and polypeptide have shown that the nucleotidyl-protein is VPg-pUpU: the genome-linked protein of poliovirion RNA covalently bound to the first two 5'-terminal nucleotides of poliovirus RNA. Synthesis of VPg-pUpU in vitro was sensitive to nonionic detergent. We suggest that VPg-pUpU is part of the initiation complex in poliovirus RNA replication in a membranous environment.
Takegami et al. (1983) studied this question.
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