What question did this study set out to answer?

Investigate how native and [14C]-carboxymethylated cytochrome c interact with, penetrate, and adsorb onto monolayers of phosphatidylcholine, phosphatidic acid, and cardiolipin.

October 1, 1969Biochemical JournalOpen Access

Interactions of cytochrome c and 14C-carboxymethylated cytochrome c with monolayers of phosphatidylcholine, phosphatidic acid and cardiolipin

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Authors

PQPeter J. QuinnSt. John's College of Nursing RDR. M. C. DawsonNorthwestern University

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Implication

In vitro monolayer study reveals preferential binding of cytochrome c to cardiolipin and phosphatidic acid, highlighting cardiolipin as the key mitochondrial lipid partner.

Key Points

Investigate how native and [14C]-carboxymethylated cytochrome c interact with, penetrate, and adsorb onto monolayers of phosphatidylcholine, phosphatidic acid, and cardiolipin.
Measured surface radioactivity, surface pressure, and surface potential at an air/water interface across variable subphase ionic strengths (10 mM versus 1 M sodium chloride) and pH levels.
Assessed penetration dynamics and adsorption reversibility of native and [14C]-carboxymethylated cytochrome c across egg phosphatidylcholine, phosphatidic acid, and cardiolipin monolayers.
Cytochrome c penetration into phosphatidylcholine monolayers was inversely proportional to initial film pressure, reached zero at 20 dynes/cm, and was virtually eliminated by monolayer compression above this threshold.
Penetration into phosphatidic acid and cardiolipin monolayers persisted up to film collapse pressures (>40 dynes/cm) and featured strong electrostatic adsorption that was substantially reversed by 1 M sodium chloride.

Cite This Study

Quinn et al. (1969) studied this question.

synapsesocial.com/papers/6a1d5bc173c56dd1bd2f92b3 https://doi.org/10.1042/bj1150065

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Implication

In vitro monolayer study reveals preferential binding of cytochrome c to cardiolipin and phosphatidic acid, highlighting cardiolipin as the key mitochondrial lipid partner.

Interactions of cytochrome c and 14C-carboxymethylated cytochrome c with monolayers of phosphatidylcholine, phosphatidic acid and cardiolipin

Discussion

Member takes

Implication

Key Points

Cite This Study

Interactions of cytochrome c and 14C-carboxymethylated cytochrome c with monolayers of phosphatidylcholine, phosphatidic acid and cardiolipin

Discussion

Member takes

Implication

Key Points

Cite This Study

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