Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
February 1, 1957Biochemical JournalOpen Access

The structure of the naturally occurring phosphoglycerides. 3. Action of moccasin-venom phospholipase A on ovolecithin and related substances

View Full Paper
Ask AI
Bookmark
Share

Authors

CLC. LongUniversity of SarajevoIPI. F. PennyUniversity of Aberdeen

Discussion

Loading...

Member takes

Implication

Biochemical analysis demonstrates enzymatic cleavage of ovolecithin by moccasin venom phospholipase A, highlighting the positional structure of natural phosphoglycerides.

Key Points

  • To determine the enzymatic cleavage mechanism of moccasin venom phospholipase A on ovolecithin and related phosphoglyceride substrates to elucidate their chemical structures.
  • Incubated ovolecithin and related phosphoglyceride compounds with phospholipase A derived from moccasin venom in vitro.
  • Separated and characterized the resulting lyso-compounds and released free fatty acids to identify specific ester bond hydrolysis.
  • Moccasin venom phospholipase A catalyzes site-specific hydrolysis of ester bonds in ovolecithin and structurally related phosphoglycerides.
  • Identification of liberated fatty acids reveals the positional distribution of acyl groups on the glycerol backbone of naturally occurring lipids.

Cite This Study

Long et al. (1957) studied this question.

synapsesocial.com/papers/6a1d5bc473c56dd1bd2f92e5https://doi.org/10.1042/bj0650382
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Phospholipids. 3. On the chromatographic separation of glycerophospholipids1955 · 326 citations
  2. 2Enzyme inhibitions by snake venoms1953 · 118 citations
  3. 3An improved method for the colorimetric determination of phosphate1938 · 1,081 citations
  4. 4Purification and properties of a lysolecithinase from pancreas1953 · 115 citations