What does this research mean for the field?

Retroviral vectors carrying two genes fail to express the second gene at levels equivalent to single-gene vectors in hemopoietic cells due to inefficient splicing and a reduction in unspliced transcripts. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

July 1, 1988Journal of VirologyOpen Access

Comparison of expression in hemopoietic cells by retroviral vectors carrying two genes

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Population

Hemopoietic cells infected with recombinant retroviral vectors

Comparison

Retroviral vectors carrying two genes utilizing… vs Parental vectors carrying only the neomycin…

Design

Preclinical

Authors

DBDavid D.L. BowtellUniversity College Dublin SCSuzanne CoryThe University of Melbourne GJGregory R. JohnsonXLIM

Discussion

Member takes

Implication

Retroviral coexpression inefficiencies in animal models warrant design caution; leaves open whether splicing optimizations enable clinical translation.

Structured PICO

Population

Hemopoietic cells infected with recombinant retroviral vectors

Intervention

Retroviral vectors carrying two genes (neomycin resistance gene and a second gene: myc, myb, or granulocyte-macrophage colony-stimulating factor) utilizing alternate splicing or a separate internal promoter

Comparator

Parental vectors carrying only the neomycin resistance gene

Outcome

Viral RNA levels and expression of the second genesurrogate

Retroviral vectors carrying two genes showed lower expression of the second gene compared to single-gene parental vectors in hemopoietic cells, highlighting challenges in vector design for coexpression.

Cite This Study

Bowtell et al. (1988) studied this question.

synapsesocial.com/papers/6a1d85c97328fa9a742fa005 https://doi.org/10.1128/jvi.62.7.2464-2473.1988

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Implication

Retroviral coexpression inefficiencies in animal models warrant design caution; leaves open whether splicing optimizations enable clinical translation.

Comparison of expression in hemopoietic cells by retroviral vectors carrying two genes

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Comparison of expression in hemopoietic cells by retroviral vectors carrying two genes

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider