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April 1, 1996Journal of Clinical InvestigationOpen Access

Activated transcription factor nuclear factor-kappa B is present in the atherosclerotic lesion.

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Key Points

To determine whether activated nuclear factor-kappa B (NF-kappaB) is present in human atherosclerotic lesions and to explore its role in vascular disease pathogenesis.
Analyzed human atherosclerotic lesions and non-atherosclerotic control vessels.
Used immunofluorescence and immunohistochemistry with a monoclonal antibody (alpha-p65mAb) specific to the activated p65 subunit of NF-kappaB.
Assessed functional activity and target gene expression using electrophoretic mobility shift assays and colocalization analyses.
Activated NF-kappaB localized to fibrotic-thickened intima/media and atheromatous regions within smooth muscle cells, macrophages, and endothelial cells.
Vessels lacking atherosclerosis displayed little to no activated NF-kappaB.

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Implication

Laboratory analysis reveals activated nuclear factor-kappa B within human atherosclerotic lesions, indicating a key role for this transcription factor in chronic vascular inflammation.

Cite This Study

Brand et al. (1996) studied this question.

synapsesocial.com/papers/6a1db85b984ea5c2d0ce322d https://doi.org/10.1172/jci118598

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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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3Localization of tissue factor in the normal vessel wall and in the atherosclerotic plaque.Original Research · 1989 · 1,228 citations
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