A new modification of SDS-PAGE allows for faster, safer, and more efficient separation of cardiac myosin heavy chains.
May streamline preclinical cardiac myosin analysis in rodents; leaves open translation to human samples.
Using our own new modification of one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) we successfully separated myosin heavy chains from different chambers of rat heart. The key features of modification are changes in the content and buffer composition of the separating gel, and employment of constant current instead of voltage. These changes allow carrying out a safer and faster one-dimensional SDS-PAGE for cardiac myosin heavy chains using significantly fewer reagents.
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Gerzen et al. (2022) studied this question.
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