Describes a simplified mini-gel SDS-PAGE protocol for separating myosin heavy-chain isoforms in bovine, ovine, and camel skeletal muscle.
May aid large-animal muscle phenotyping; leaves open translation to human cardiac or clinical use.
In this short communication we describe a specific protocol for SDS-PAGE separation of adult bovine myosin heavy-chain (MyHC) isoforms. The conditions defined in this protocol allow a good separation with a good reproducibility of the four MyHC isoforms (MyHC I, IIa, IIx, IIb) identified in adult skeletal muscle of this species. This procedure uses mini-gel electrophoresis system and does not involve preparation of gradient separating gels. In addition, this protocol can also be applied to the electrophoretic separation of ovine and camel MyHC isoforms.
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Picard et al. (2011) studied this question.
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