Why the study?
Does a new ELISA method accurately determine muscle myosin heavy chain isoforms compared to the established SDS-PAGE method in rat muscle samples?
Does a new ELISA method accurately determine muscle myosin heavy chain isoforms compared to the established SDS-PAGE method in rat muscle samples?
A newly developed ELISA method for determining myosin heavy chain isoforms provides semi-quantitative results comparable to SDS-PAGE but is not superior in accuracy or speed.
ELISA supports semi-quantitative MyHC analysis in animal muscle; leaves open superiority over SDS-PAGE for research or clinical translation.
We developed a new method for the quantitative determination of myosin heavy chain (MyHC) isoforms taking advantage of immunochemical differences and based on the ELISA principle. In the present paper we compare analysis of MyHC isoforms using the SDS-PAGE and the ELISA methods in the same samples of adult female inbred Lewis strain euthyroid, hyperthyroid and hypothyroid rats. In all thyroid states, the same composition and corresponding changes of MyHC isoforms were determined using both methodological approaches in the slow soleus and the fast extensor digitorum longus muscles. Our results showed that ELISA can be used for a "semi-quantitative" or "comparative" measurement of MyHC isoforms in multiple muscle samples, but that it is neither more exact nor faster compared to SDS-PAGE.
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Říčný et al. (2011) studied this question.
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