Key result
Cardiac MyHC α shows higher electrophoretic mobility than MyHC β on SDS-PAGE.
Why the study?
Does thyroid status alter the expression and electrophoretic mobility of cardiac MyHC isoforms in rat left ventricles?
Does thyroid status alter the expression and electrophoretic mobility of cardiac MyHC isoforms in rat left ventricles?
This study demonstrates that cardiac MyHC α has a higher electrophoretic mobility than MyHC β on SDS-PAGE, challenging previous literature and highlighting the need for careful identification of these isoforms.
Requires re-evaluation of MyHC isoform mobility on SDS-PAGE in rat models; challenges consensus and leaves open translation to human cardiac research.
The expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats. Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS‐PAGE, whereas hypothyroid LVs predominantly contained a MyHC isoform with a lower mobility corresponding to that of the control soleus muscle. By comparing the MyHC profiles obtained under altered thyroid states together with the control soleus, we concluded that MyHC α was represented by the lower band with higher mobility and MyHC β by the upper band. The identity of these two bands in SDS‐PAGE gels was confirmed by western blot and mass spectrometry. Thus, in contrast to the literature data, we found that the MyHC α possessed a higher mobility rate than the MyHC β isoform. Our data highlighted the importance of the careful identification of the MyHC α and MyHC β isoforms analyzed by the SDS‐PAGE.
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Arnostova et al. (2011) studied Altered thyroid status (hyperthyroidism and hypothyroidism). Altered thyroid status (T3 injections or methimazole) vs. Euthyroid age-matched littermates was evaluated on Electrophoretic mobility of cardiac MyHC α and MyHC β isoforms on SDS-PAGE. Contrary to prevailing literature, the cardiac MyHC α isoform possessed a higher electrophoretic mobility rate on SDS-PAGE than the MyHC β isoform in rat left ventricles.
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