Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
December 15, 2022Frontiers in MicrobiologyOpen Access

Assessment of the accuracy of 11 different diagnostic tests for the detection of Schistosomiasis mansoni in individuals from a Brazilian area of low endemicity using latent class analysis

View Full Paper
Ask AI
Bookmark
Share

Authors

SMSilvia Gonçalves MesquitaRCRoberta Lima CaldeiraTFTereza Cristina Favre

Discussion

Loading...

Member takes

Overview

Cross-sectional study demonstrates high accuracy for two-step testing combining ELISA and stool NAATs in low-endemicity populations, suggesting cost-effective strategies to eliminate schistosomiasis.

Key Points

  • To evaluate the diagnostic accuracy of 11 parasitological, molecular, and immunological tests for detecting Schistosoma mansoni in a low-endemicity setting using latent class analysis.
  • Conducted a cross-sectional survey analyzing stool, urine, and blood samples from 400 individuals aged >6 years untreated with praziquantel in the prior 12 months in Malacacheta, Minas Gerais, Brazil.
  • Evaluated 11 assays: Kato-Katz parasitological examination; urine NAATs (PCR, qPCR, LAMP); stool NAATs (PCR-ELISA, qPCR, LAMP); POC-CCA; commercial IgG ELISA; and two in-house ELISAs.
  • Applied latent class analysis in the absence of a definitive gold standard to estimate disease prevalence alongside assay sensitivity and specificity.
  • Latent class analysis estimated a schistosomiasis prevalence of 12% (95% CI: 9–15%), while single-test positivity rates ranged from 5.0% for urine qPCR to 40.8% for commercial ELISA.
  • The commercial ELISA achieved the highest sensitivity (100%), whereas Kato-Katz demonstrated the highest specificity (99%).
  • Proposed two-step diagnostic strategies combining commercial ELISA screening with stool NAAT confirmation exceeded the mean sensitivity (70.4%) and specificity (89.5%) of the 11 individual tests.

Cite This Study

Mesquita et al. (2022) studied this question.

synapsesocial.com/papers/6a1e6b9b40bc8a3dd768dcf3https://doi.org/10.3389/fmicb.2022.1048457
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Validation and evaluation of diagnostic tests for Schistosoma mansoni in Brazil: The ValidaXisto study protocol2026
  2. 2Comparative performance of Kato-Katz and spontaneous sedimentation methods for schistosomiasis mansoni diagnosis in endemic areas of Northeastern Brazil2026
  3. 3Diagnostic Accuracy and Applicability of a PCR System for the Detection of Schistosoma mansoni DNA in Human Urine Samples from an Endemic Area2012 · 86 citations
  4. 4Diagnostic Performance and Discordance of Kato–Katz Method, POC-CCA Test, and PCR in Detecting Schistosoma mansoni in a Low-Prevalence South African Setting2026
  5. 5Accuracy of commercial ELISA and ICT for screening schistosomiasis infections at a low endemicity area in Brazil2024 · 2 citations