A Reduction in ADAM17 Expression Is Involved in the Protective Effect of the PPAR-α Activator Fenofibrate on Pressure Overload-Induced Cardiac Hypertrophy

Figures

• 

  Figure 1. Fenofibrate inhibited pressure-overload-induced cardiac hypertrophy in abdominal artery constriction- (AAC-) induced hypertensive rats. (a) HW/BW; (b) LVW/BW; (c) LVAW; (d) LVPW; (e) ANP mRNA level; (f) BNP mRNA level. Feno represents fenofibrate; HW/BW represents ratio between heart weight and body weight; LVW/BW represent ratio between left ventricular weight and body weight; LVAW represents left ventricular anterior wall thickness; LVPW represents left ventricular posterior wall thickness; #p<0.05 versus Sham group; ∗p<0.05 versus AAC group, n=6 per group.

• 

  Figure 2. Fenofibrate reduced the expression of a disintegrin and metalloproteinase-17 (ADAM17) in left ventricular tissue from abdominal artery constriction- (AAC-) induced hypertensive rats. (a) ADAM17 protein level (n=4 independent experiments); (b) ADAM17 mRNA level (n=3 independent experiments). Feno represents fenofibrate; #p<0.05 versus Sham group; ∗p<0.05 versus AAC group.

• 

  Figure 3. Fenofibrate attenuated angiotensin II-induced cardiac hypertrophy in cultured primary cardiomyocytes. After pretreated with fenofibrate (10 μM) for 1 hour, cardiomyocytes were then stimulated with 100 nM angiotensin II for 24 hours. (a) Cell surface area; (b) ANP mRNA level; (c) BNP mRNA level. Ang II represents angiotensin II; Feno represents fenofibrate; #p<0.05 versus control group; ∗p<0.05 versus Ang II group. n=3 independent experiments.

• 

  Figure 4. Fenofibrate reduced the expression of a disintegrin and metalloproteinase-17 (ADAM17) in primary cardiomyocytes stimulated with 100 nM angiotensin II for 24 hours. After pretreated with fenofibrate (10 μM) for 1 hour, cardiomyocytes were then stimulated with 100 nM angiotensin II for 24 hours. (a) ADAM17 protein level (n=4 independent experiments); (b) ADAM17 mRNA level (n=3 independent experiments). Ang II represents angiotensin II; Feno represents fenofibrate; #p<0.05 versus control group; ∗p<0.05 versus Ang II group.