What question did this study set out to answer?

To determine the identity and chemical linkage of the fatty acid incorporated into endothelial nitric oxide synthase (eNOS).

April 1, 1994Journal of Biological ChemistryOpen Access

Identification of covalently bound amino-terminal myristic acid in endothelial nitric oxide synthase.

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Authors

JLJ LiuYale University WSWilliam C. SessaVascular Medicine

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Implication

Biochemical study reveals covalent myristic acid incorporation into endothelial nitric oxide synthase in endothelial and transfected cells, indicating a co-translational membrane-anchoring...

Key Points

To determine the identity and chemical linkage of the fatty acid incorporated into endothelial nitric oxide synthase (eNOS).
Metabolically labeled wild-type or G2A-mutant eNOS-transfected COS cells and bovine aortic endothelial cells with [3H]myristic acid for 5 hours.
Assessed radiolabel linkage sensitivity to hydroxylamine and evaluated the necessity of protein synthesis using cycloheximide.
Conducted chemical analysis of released fatty acids following acid methanolysis of labeled eNOS.
[3H]myristic acid incorporated selectively into membrane-associated wild-type eNOS and endothelial cells, but not into the G2A mutant despite equivalent protein expression levels.
Radiolabel linkage to eNOS was resistant to hydroxylamine treatment and was completely blocked by cycloheximide, confirming an amide linkage formed during translation.
Acid methanolysis verified that the released radiolabel was authentic protein-bound myristic acid.

Cite This Study

Liu et al. (1994) studied this question.

synapsesocial.com/papers/6a1ea83e989adebfe89a6abd https://doi.org/10.1016/s0021-9258(17)32626-1

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Implication

Biochemical study reveals covalent myristic acid incorporation into endothelial nitric oxide synthase in endothelial and transfected cells, indicating a co-translational membrane-anchoring...

Identification of covalently bound amino-terminal myristic acid in endothelial nitric oxide synthase.

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Implication

Key Points

Cite This Study

Identification of covalently bound amino-terminal myristic acid in endothelial nitric oxide synthase.

Discussion

Member takes

Implication

Key Points

Cite This Study

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