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January 1, 1995Journal of Biological ChemistryOpen Access

Agonist-modulated Palmitoylation of Endothelial Nitric Oxide Synthase

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Population

Endothelial cells (transiently transfected and biosynthetically labeled)

Design

Preclinical

Authors

LRLisa RobinsonHarvard UniversityLBLiliana BusconiBoston UniversityTMThomas MichelCross-Cutting Cardiology

Discussion

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Implication

Cell study suggests novel ecNOS regulation; leaves open in vivo validation and clinical relevance.

Key Points

  • This research aims to explore how agonists like bradykinin affect the palmitoylation and translocation of endothelial nitric oxide synthase (ecNOS).
  • Transient transfection of endothelial cells was employed to study ecNOS modifications.
  • Biosynthetic labeling with [3H]palmitic acid was used to examine palmitoylation of the enzyme.
  • Pulse-chase experiments assessed the effects of bradykinin on ecNOS depalmitoylation.
  • ecNOS is found to be reversibly palmitoylated as confirmed by hydroxylamine release and acid hydrolysis.
  • Bradykinin treatment promotes depalmitoylation of ecNOS, facilitating its translocation to the cytosol.
  • Phosphorylation of ecNOS occurs even in a myristoylation-deficient context, indicating a specific regulatory mechanism.

Structured PICO

P
Population
Endothelial cells (transiently transfected and biosynthetically labeled)
I
Intervention
Bradykinin
O
Outcome
ecNOS palmitoylation status and subcellular localizationsurrogate

This study demonstrates that endothelial nitric oxide synthase is reversibly palmitoylated and that bradykinin promotes its depalmitoylation, regulating its subcellular localization.

Cite This Study

Robinson et al. (1995) studied this question.

synapsesocial.com/papers/6a75690f756843cacbb1ff45https://doi.org/10.1074/jbc.270.3.995
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Endothelial nitric oxide synthase. N-terminal myristoylation determines subcellular localization.1993 · 276 citations
  2. 2Phosphorylation and subcellular translocation of endothelial nitric oxide synthase.1993 · 325 citations
  3. 3Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform.1992 · 993 citations
  4. 4Agonist-modulated palmitoylation of beta 2-adrenergic receptor in Sf9 cells.1992 · 177 citations
  5. 5Dual myristylation and palmitylation of Src family member p59fyn affects subcellular localization1994 · 211 citations