Key result
Activation of the protein kinase C signaling pathway with TPA reversed the intrinsic chiral rotational bias of cardiac cells, reducing rightward cardiac looping from 80% to 10% in chicken embryos.
Why the study?
Biophysical mechanisms underlying left-right asymmetry remain unclear, particularly the role of intrinsic cell chirality on organ asymmetry during cardiac C looping.
Population
Developing chicken embryos and isolated chick cardiac cells
Comparison
Reversal of cell chirality via protein kinase C pathway activation vs control
Design
Preclinical in vitro and in vivo developmental biology study
Authors
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May inform mechanisms of congenital heart defects; hypothesis-generating in avian models, human relevance unknown.
Absolute Event Rate: 10% vs 80%
p-value: p=<0.001
Intrinsic cellular chirality regulates left-right symmetry breaking during cardiac looping, and activation of PKC signaling reverses this chirality and the directionality of cardiac looping.
Ray et al. (2018) studied Cardiac looping (embryonic development). Protein kinase C (PKC) activation (TPA) vs. Control (untreated/DMSO) was evaluated on Rightward cardiac looping (p=<0.001). Activation of the protein kinase C signaling pathway with TPA reversed the intrinsic chiral rotational bias of cardiac cells, reducing rightward cardiac looping from 80% to 10% in chicken embryos.
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