What question did this study set out to answer?

To develop a universal, degenerate primer-based RT-PCR screening assay capable of detecting all known and emerging members of the Coronaviridae family.

January 1, 2008SpringerOpen Access

A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses

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To develop a universal, degenerate primer-based RT-PCR screening assay capable of detecting all known and emerging members of the Coronaviridae family.
Designed degenerate primers directed at a conserved region of the coronavirus RNA polymerase gene.
Established an RT-PCR protocol followed by sequencing of the resulting 251-bp amplicon to confirm and identify viral species.
The degenerate RT-PCR assay amplifies a conserved 251-bp fragment across diverse coronaviruses causing mild to severe respiratory illnesses.
Sequencing of the amplified polymerase gene segment enables identification of both established strains (including SARS-CoV, HCoV-NL63, and HCoV-HKU1) and novel viral variants.

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Implication

Methodological protocol demonstrates broad-spectrum RT-PCR detection targeting the coronavirus polymerase gene, facilitating surveillance of known and novel respiratory viruses.

Cite This Study

Vijgen et al. (2008) studied this question.

synapsesocial.com/papers/6a1eec11f26b1ca3a088cb53 https://doi.org/10.1007/978-1-59745-181-9_1

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Implication

Methodological protocol demonstrates broad-spectrum RT-PCR detection targeting the coronavirus polymerase gene, facilitating surveillance of known and novel respiratory viruses.

A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses

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Discussion

Implication

Cite This Study

A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses

Key Points

Discussion

Implication

Cite This Study

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