Key result
Overexpression of the Z1.1GFP fusion protein in living muscle cells led to a dominant negative phenotype characterized by myofibril disassembly as titin-GFP fragments accumulated.
Population
Cultures of nonmuscle cells, skeletal myotubes, and cardiomyocytes
Design
Preclinical
Authors
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Implicates titin Z-band in myofibril stability; hypothesis-generating for sarcomeric cardiomyopathies but requires mammalian validation before guiding research or practice.
The Z-band region of titin is crucial for maintaining and organizing myofibril structure in living muscle cells.
Turnacioglu et al. (1997) studied this question. Z1.1GFP fusion construct (N-terminal fragment of titin coupled to GFP) vs. Untransfected cells or cells expressing GFP alone was evaluated on Localization of Z1.1GFP and effect of overexpression on myofibril structure. Overexpression of the Z1.1GFP fusion protein in living muscle cells led to a dominant negative phenotype characterized by myofibril disassembly as titin-GFP fragments accumulated.
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