What is the clinical evidence from this study?

Intervention: Removal of thin filaments with plasma gelsolin. Primary outcome: Identification of elastic filaments and their protein component.

What question did this study set out to answer?

Investigate the structural identity and mechanical function of elastic filaments in skeletal muscle following the selective removal of thin filaments.

January 1, 1990The Journal of Cell BiologyOpen Access

Elastic filaments in skeletal muscle revealed by selective removal of thin filaments with plasma gelsolin.

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Key result

Selective removal of thin filaments in rabbit skeletal muscle revealed that the main protein component of the elastic filaments connecting thick filaments and the Z line is alpha-connectin (titin 1).

Key Points

Investigate the structural identity and mechanical function of elastic filaments in skeletal muscle following the selective removal of thin filaments.
Extracted thin filaments from rabbit skeletal muscle fibers using plasma gelsolin, confirming removal via phalloidin-rhodamine fluorescence microscopy, myosin S1 arrowhead decoration in electron microscopy, and SDS-PAGE.
Visualized filament architecture using thin-section electron microscopy while evaluating active tension, rigor stiffness, and resting tension in thin filament-free fibers.
Treated thin filament-free fibers with mild trypsin digestion to assess the parallel degradation of remaining mechanical tension and muscle structural proteins.
Removal of thin filaments abolished active tension generation and rigor stiffness while fully maintaining resting tension, revealing approximately 4 nm diameter elastic filaments linking thick filaments directly to the Z line.
Mild trypsin digestion eliminated resting tension and degraded elastic filaments in direct correlation with the proteolytic cleavage of alpha-connectin (titin 1) into beta-connectin (titin 2).

Structured PICO

Population

Rabbit skeletal muscle fibers

Intervention

Selective removal of thin filaments with plasma gelsolin and subsequent mild trypsin treatment

Outcome

Identification and characterization of elastic filaments and their protein composition

The study demonstrates that alpha-connectin (titin 1) is the main protein component of the elastic filaments in skeletal muscle, providing passive elasticity.

Discussion

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Implication

Identifies titin as skeletal elastic filament protein; leaves open its role in cardiac passive tension and HF.

Cite This Study

Funatsu et al. (1990) studied this question. Removal of thin filaments with plasma gelsolin was evaluated on Identification of elastic filaments and their protein component. Selective removal of thin filaments in rabbit skeletal muscle revealed that the main protein component of the elastic filaments connecting thick filaments and the Z line is alpha-connectin (titin 1).

synapsesocial.com/papers/6a1eee2d5983aaf3a280a60d https://doi.org/10.1083/jcb.110.1.53

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Elastic filaments in skeletal muscle revealed by selective removal of thin filaments with plasma gelsolin.

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Elastic filaments in skeletal muscle revealed by selective removal of thin filaments with plasma gelsolin.

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

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