What question did this study set out to answer?

Investigate how glycolytic inhibitors, intermediates, and metabolic end products modulate sarcoplasmic reticulum calcium release and uptake in cardiac atrial myocytes.

February 4, 2005The Journal of PhysiologyOpen Access

Modulation of sarcoplasmic reticulum Ca²⁺ release by glycolysis in cat atrial myocytes

View Full Paper

Ask AI

Bookmark

Investigate how glycolytic inhibitors, intermediates, and metabolic end products modulate sarcoplasmic reticulum calcium release and uptake in cardiac atrial myocytes.
Studied intact and permeabilized cat atrial myocytes under field stimulation (0.5–0.7 Hz) and focal inhibitor exposure to track intracellular calcium transients and calcium sparks.
Measured single-channel open probability of ryanodine receptors incorporated into planar lipid bilayers across various glycolytic intermediates and products (1 mM each).
Inhibition of glycolysis using 2-deoxy-D-glucose (10 mM) or iodoacetate (1 mM) triggered calcium alternans, slowed transient decay, and reduced sarcoplasmic reticulum calcium load by 15% to 20%.
Single ryanodine receptor recordings showed that fructose-6-phosphate increased open probability by 40% and fructose-1,6-bisphosphate increased it by 265%, whereas pyruvate decreased it by 58%.
Spontaneous calcium sparks in permeabilized cells were enhanced by fructose-1,6-bisphosphate and suppressed by pyruvate, while mitochondrial redox state remained largely unaffected by glycolytic inhibition.

Structured PICO

Population

Intact and permeabilized cat atrial myocytes, and ryanodine receptors (RyRs) incorporated into lipid bilayers

Intervention

Inhibitors (2-deoxy-D-glucose (2-DG), iodoacetate (IAA)), intermediates (glucose-6-phosphate (G6P), fructose-6-phosphate (F6P), fructose-1,6-bisphosphate (FBP), phosphoenolpyruvate (PEP)) and products (pyruvate, L-lactate) of glycolysis

Outcome

Sarcoplasmic reticulum (SR) Ca2+ release and uptakesurrogate

In cat atrial myocytes, glycolysis regulates SR Ca2+ release through direct modulation of RyR activity by glycolytic intermediates and products, and modulation of SERCA activity via local ATP changes.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

No immediate clinical implications; leaves open glycolytic modulation of SR Ca2+ release in human hearts.

Cite This Study

Kockskämper et al. (2005) studied this question.

synapsesocial.com/papers/6a1ef6f01baba023eb6b57c4 https://doi.org/10.1113/jphysiol.2004.078782

View Full Paper

Ask AI

Bookmark

Modulation of sarcoplasmic reticulum Ca²⁺ release by glycolysis in cat atrial myocytes

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Modulation of sarcoplasmic reticulum Ca²⁺ release by glycolysis in cat atrial myocytes

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider