What is the clinical evidence from this study?

Study design: Other. Intervention: Acidosis vs. Baseline/Normal pH. Primary outcome: Ca2+ uptake and release by the sarcoplasmic reticulum.

What question did this study set out to answer?

This research investigates how acidosis influences calcium uptake and release in rat ventricular myocytes.

September 1, 1998AJP Heart and Circulatory Physiology

Effect of acidosis on Ca2+uptake and release by sarcoplasmic reticulum of intact rat ventricular myocytes

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Key result

Acidosis inhibits sarcoplasmic reticulum Ca2+ uptake and Ca2+-induced Ca2+ release in intact rat ventricular myocytes, but these effects are compensated by an increase in SR Ca2+ content.

Population

intact rat ventricular myocytes

Design

Preclinical

Authors

JHJoanne T. HulmeRadiant Research (United States)COClive H. OrchardElectrophysiology

Discussion

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Implication

Hypothesis-generating for Ca²⁺ dysregulation in acidotic hearts; extends rat myocyte data but leaves clinical translation open.

Key Points

This research investigates how acidosis influences calcium uptake and release in rat ventricular myocytes.
Monitored intracellular calcium concentrations using fura 2.
Used perforated patch-clamp technique to assess L-type calcium current.
Induced acidosis through acid solution superfusion and NH4Cl withdrawal.
Acidosis increased the amplitude and slowed the declining phase of calcium transients.
Caffeine-induced rise in intracellular calcium decreased with sustained acid, but NH4Cl withdrawal had no effect.
Acidosis reduced the fraction of caffeine-induced calcium released by electrical stimulation.

Structured PICO

Population

intact rat ventricular myocytes

Intervention

acidosis (produced by superfusing cells with an acid solution for intracellular and extracellular acidosis, or by NH4Cl withdrawal for intracellular acidosis)

Outcome

Ca2+ uptake and release by the sarcoplasmic reticulum (SR)surrogate

In intact rat ventricular myocytes, acidosis inhibits sarcoplasmic reticulum Ca2+ uptake and release, which is compensated by increased SR Ca2+ content due to elevated cytoplasmic Ca2+.

Cite This Study

Hulme et al. (1998) studied this question. Acidosis vs. Baseline/Normal pH was evaluated on Ca2+ uptake and release by the sarcoplasmic reticulum. Acidosis inhibits sarcoplasmic reticulum Ca2+ uptake and Ca2+-induced Ca2+ release in intact rat ventricular myocytes, but these effects are compensated by an increase in SR Ca2+ content.

synapsesocial.com/papers/6a62eec53eb69703d1b6e147 https://doi.org/10.1152/ajpheart.1998.275.3.h977

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Effect of acidosis on Ca2+uptake and release by sarcoplasmic reticulum of intact rat ventricular myocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effect of acidosis on Ca2+uptake and release by sarcoplasmic reticulum of intact rat ventricular myocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider