What question did this study set out to answer?

Assess the impact of CO2-induced acidosis on contraction and ion concentrations in rat ventricular myocytes.

February 1, 1992AJP Cell Physiology

Contraction and intracellular Ca2+, Na+, and H+ during acidosis in rat ventricular myocytes

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Why the study?

Does CO2-induced acidosis affect contraction and intracellular ions in rat ventricular myocytes?

Population

Ventricular myocytes isolated from rat hearts

Comparison

CO2-induced (respiratory) acidosis vs Baseline/normal conditions

Design

Preclinical

Authors

SHSimon M. HarrisonCommonwealth Scientific and Industrial Research Organisation JFJames E. FramptonHammersmith Hospital EMEileen McCallAstraZeneca (Brazil)

Discussion

Member takes

Implication

Rat myocyte data implicate Na+-H+ exchange in acidosis recovery; leaves open relevance to human ventricular function.

Key Points

Assess the impact of CO2-induced acidosis on contraction and ion concentrations in rat ventricular myocytes.
Isolated rat ventricular myocytes were exposed to CO2-induced acidosis.
Intracellular Ca2+, Na+, and pH were measured using fluorescent dyes.
Effects of Na(+)-H+ exchange inhibitor amiloride and sarcoplasmic reticulum inhibitor ryanodine were evaluated.
Initial exposure to acidosis caused a rapid decrease in intracellular pH and contractility.
Recovery of contraction was accompanied by increased intracellular Na+ and Ca2+ following the initial decline.
Na(+)-H+ exchange and sarcoplasmic reticulum mechanisms mediated the recovery process.

Structured PICO

Does CO2-induced acidosis affect contraction and intracellular ions in rat ventricular myocytes?

Population

Ventricular myocytes isolated from rat hearts

Intervention

CO2-induced (respiratory) acidosis

Comparator

Baseline/normal conditions

Outcome

Contraction and intracellular Ca2+, Na+, and pHsurrogate

In rat ventricular myocytes, acidosis initially depresses contractility via decreased intracellular pH, followed by a partial recovery mediated by Na+-H+ exchange activation leading to increased intracellular Na+ and Ca2+.

Cite This Study

Harrison et al. (1992) studied this question.

synapsesocial.com/papers/6a6f3f00e5469ee92be044a4 https://doi.org/10.1152/ajpcell.1992.262.2.c348

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Contraction and intracellular Ca2+, Na+, and H+ during acidosis in rat ventricular myocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Contraction and intracellular Ca2+, Na+, and H+ during acidosis in rat ventricular myocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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