What question did this study set out to answer?

To investigate how endothelial cells influence neutrophil adhesion and the role of lipoxygenase metabolites in this process.

June 1, 1993Journal of Leukocyte Biology

Human neutrophil adhesion to bovine aortic endothelium. Evidence for endothelial lipoxygenase activity

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To investigate how endothelial cells influence neutrophil adhesion and the role of lipoxygenase metabolites in this process.
Cultured bovine aortic endothelial cells preincubated with various inflammatory signals, including phorbol myristate acetate.
Neutrophil adhesion assessed using 51Cr-labeled neutrophils after treatment with lipoxygenase inhibitors.
Endothelium analyzed for production of fatty acid metabolites indicative of lipoxygenase activity.
Endothelial preincubation with phorbol myristate acetate increased neutrophil adhesion by 5.7-fold.
Lipoxygenase inhibitors significantly reduced phorbol-induced adhesion by 55% (nordihydroguaiaretic acid) and 27% (5,8,11,14-eicosatetraenoic acid).
Leukotriene B4 was identified as a product of endothelial lipoxygenase and enhanced neutrophil adhesion.

Structured PICO

Population

Cultured bovine aortic endothelial cells and 51Cr-labeled neutrophils

Intervention

Preincubation with phorbol myristate acetate, A23187, thrombin, f-Met-Leu-Phe, and lipoxygenase inhibitors (nordihydroguaiaretic acid, 5,8,11,14-eicosatetraenoic acid, BW755C)

Comparator

Baseline/untreated endothelial cells

Outcome

Neutrophil adherence to endothelial cellssurrogate

Endothelial cell-derived lipoxygenase metabolites, particularly LTB4, are involved in the acute regulation of neutrophil adhesion to endothelium induced by inflammatory signals.

Discussion

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Implication

Hypothesis-generating in animal endothelium; leaves open clinical relevance of lipoxygenase metabolites for human neutrophil adhesion.

Cite This Study

Damtew et al. (1993) studied this question.

synapsesocial.com/papers/6a1f620ffe2692e41a2ac6fc https://doi.org/10.1002/jlb.53.6.619

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Human neutrophil adhesion to bovine aortic endothelium. Evidence for endothelial lipoxygenase activity

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Human neutrophil adhesion to bovine aortic endothelium. Evidence for endothelial lipoxygenase activity

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider