What does this research mean for the field?

Basal adhesion of polymorphonuclear leukocytes to vascular endothelial cells is independent of cyclooxygenase derivatives and exogenous prostacyclin, but is significantly reduced by inhibitors of non-cyclooxygenase-dependent arachidonic acid metabolism. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

October 1, 1983BloodOpen Access

Arachidonic acid metabolism and the adhesion of human polymorphonuclear leukocytes to cultured vascular endothelial cells

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Population

In vitro model using cultured bovine aortic endothelial cells and radiolabeled human polymorphonuclear…

Comparison

Pretreatment with cyclooxygenase inhibitors… vs Basal conditions

Design

Preclinical

Follow-up

30 min

Authors

MBMichael R. BuchananJames Madison University MVMJ VazquezBrigham and Women's Hospital MGMA Jr GimbroneMario Negri Institute for Pharmacological Research

Discussion

Member takes

Implication

Basal PMN adhesion appears COX-independent in vitro; leaves open non-COX arachidonic acid pathways as potential inflammation targets pending in vivo confirmation.

Structured PICO

Population

In vitro model using cultured bovine aortic endothelial cells and radiolabeled human polymorphonuclear leukocytes (PMN)

Intervention

Pretreatment with cyclooxygenase inhibitors (100 microM aspirin or 1-5 microM indomethacin), PGI2 (10(-9)-10(6) M), 5,8,11,14- or 4,7,10,13-eicosatetraynoic acid, 0.5-5 mM sodium salicylate, or 10-1,000 microM indomethacin

Comparator

Basal conditions (absence of exogenous activating stimuli)

Outcome

Number of adherent leukocytes per square millimeter of endothelial surfacesurrogate

Basal PMN-endothelial adhesion in vitro is not dependent on endogenous cyclooxygenase derivatives but is reduced by agents interfering with non-cyclooxygenase-dependent arachidonic acid metabolism.

Cite This Study

Buchanan et al. (1983) studied this question.

synapsesocial.com/papers/6a1d67fc7328fa9a742f7438 https://doi.org/10.1182/blood.v62.4.889.889

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Implication

Basal PMN adhesion appears COX-independent in vitro; leaves open non-COX arachidonic acid pathways as potential inflammation targets pending in vivo confirmation.

Arachidonic acid metabolism and the adhesion of human polymorphonuclear leukocytes to cultured vascular endothelial cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Arachidonic acid metabolism and the adhesion of human polymorphonuclear leukocytes to cultured vascular endothelial cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider