What question did this study set out to answer?

To determine the incidence, velocity, and intercellular propagation characteristics of cardiac calcium waves in the intact rat heart.

May 26, 2000Circulation ResearchOpen Access

Three Distinct Types of Ca ²⁺ Waves in Langendorff-Perfused Rat Heart Revealed by Real-Time Confocal Microscopy

View Full Paper

Ask AI

Bookmark

Population

Fluo 3-loaded rat whole hearts (Langendorff-perfused)

Comparison

Real-time laser scanning confocal microscopy to… vs Comparison between intact regions…

Design

Preclinical

Authors

TKTomoyuki KanekoThe University of Tokyo HTHideo TanakaKyoto Prefectural University of Medicine MOMasahito OyamadaFuji Women's University

Discussion

Member takes

Implication

Overloaded Ca2+ waves may promote arrhythmias in animal hearts; extends mechanistic insights but leaves open human translation.

Key Points

To determine the incidence, velocity, and intercellular propagation characteristics of cardiac calcium waves in the intact rat heart.
Examined subepicardial myocardium of fluo 3-loaded, Langendorff-perfused whole rat hearts using real-time laser scanning confocal microscopy.
Assessed calcium wave behavior and propagation during sinus rhythm with 2 mmol/L extracellular calcium and during quiescence across intact and calcium-overloaded tissue regions.
Quiescent intact myocardium exhibited sporadic calcium waves at 3.8 waves·min⁻¹·cell⁻¹ with an 84 µm/s velocity, 0.16-second decline half-time, and rare intercellular propagation (ratio <0.06).
Calcium-overloaded regions (113% fluorescence vs intact) generated waves at 28 waves·min⁻¹·cell⁻¹ with a 116 µm/s velocity, 0.41-second decline half-time, and occasional intercellular spread (ratio = 0.23).
Severely overloaded regions (124% fluorescence) produced agonal waves at 133 waves·min⁻¹·cell⁻¹ with negligible intercellular propagation.

Structured PICO

Population

Fluo 3-loaded rat whole hearts (Langendorff-perfused)

Intervention

Real-time laser scanning confocal microscopy to analyze Ca2+ waves

Comparator

Comparison between intact regions, Ca2+-overloaded regions, and regions with much higher fluorescent intensity

Outcome

Incidence, propagation velocity, and intercellular propagation of Ca2+ wavessurrogate

The spatiotemporal properties of Ca2+ waves in the intact heart are diverse and modulated by the Ca2+-loading state, with overloaded and agonal waves potentially inducing contractile failure and arrhythmias.

Cite This Study

Kaneko et al. (2000) studied this question.

synapsesocial.com/papers/6a1f65d3d09bc027e4836851 https://doi.org/10.1161/01.res.86.10.1093

View Full Paper

Ask AI

Bookmark

Three Distinct Types of Ca ²⁺ Waves in Langendorff-Perfused Rat Heart Revealed by Real-Time Confocal Microscopy

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Three Distinct Types of Ca ²⁺ Waves in Langendorff-Perfused Rat Heart Revealed by Real-Time Confocal Microscopy

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider