Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
June 1, 1996Epidemiology and InfectionOpen Access

Outbreaks of Norwalk-like virus-associated gastroenteritis traced to shellfish: coexistence of two genotypes in one specimen

View Full Paper
Ask AI
Bookmark
Share

Key result

RT-PCR analysis of specimens from gastroenteritis outbreaks traced to shellfish revealed the coexistence of two distinct Norwalk-like virus genotypes within single stool and oyster specimens.

Population

Specimens of faeces, vomit, and uneaten oysters from outbreaks of acute non-bacterial gastroenteritis…

Design

Other

Authors

MSMasaaki SugiedaShizuoka Institute of Science and TechnologyKNK. NakajimaFujita Health UniversitySNSetsuko NakajimaYamagata University

Discussion

Loading...

Member takes

Implication

Supports molecular tracing of shellfish in norovirus outbreaks; leaves open the clinical impact of mixed-genotype infections.

Study Design

Type

Observational (n=25)

Structured PICO

P
Population
25 specimens of faeces, vomit, and oysters from outbreaks of acute non-bacterial gastroenteritis suspected to be caused by shellfish in Shizuoka prefecture, Japan between 1987-94.
E
Exposure
RT-PCR and nucleotide sequencing of the RNA polymerase region of Norwalk-like viruses.
O
Outcome
Nucleotide sequences and genotypes of Norwalk-like viruses in the specimens.surrogate

The study provides direct molecular evidence linking oysters to Norwalk-like virus gastroenteritis outbreaks and demonstrates the coexistence of two viral genotypes within single clinical and environmental specimens.

Limitations

  • Difficulty of finding universal primers for the SRSV viruses
  • Detailed analysis of a specific PCR band was not yet completed

Cite This Study

Sugieda et al. (1996) conducted an observational in Acute non-bacterial gastroenteritis (n=25). Norwalk-like virus exposure from shellfish was evaluated on Detection and genotyping of Norwalk-like virus sequences. RT-PCR analysis of specimens from gastroenteritis outbreaks traced to shellfish revealed the coexistence of two distinct Norwalk-like virus genotypes within single stool and oyster specimens.

synapsesocial.com/papers/6a1fc8052be0c1f4e3bb417ahttps://doi.org/10.1017/s0950268800052663
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Sequence diversity of small, round-structured viruses in the Norwalk virus group1994 · 244 citations
  2. 2Detection of enteric viruses in oysters by using the polymerase chain reaction1993 · 193 citations
  3. 3Sequence and Genome Organization of a Human Small Round-Structured (Norwalk-Like) Virus1993 · 380 citations
  4. 4Winter Vomiting Disease1969 · 348 citations
  5. 5Western blot (immunoblot) assay of small, round-structured virus associated with an acute gastroenteritis outbreak in Tokyo1989 · 49 citations