What is the clinical evidence from this study?

Study design: Cross-Sectional. Population: Gastroenteritis (n=395). Intervention: Reverse transcription-polymerase chain reaction (RT-PCR). Primary outcome: Detection of Norwalk-like viruses.

April 1, 1996Microbiology and ImmunologyOpen Access

Detection and Sequencing of Norwalk‐Like Viruses from Stool Samples in Japan Using Reverse Transcription‐Polymerase Chain Reaction Amplification

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Key result

Norwalk-like viruses were detected by RT-PCR in 7% of all sporadic gastroenteritis cases and 15 of 17 outbreak cases in Japan, with two subgenogroups identified.

Population

378 stool samples from patients with gastroenteritis in an outpatient clinic of a hospital from 1991 to…

Design

Cross-sectional

Authors

MNMiyuki NakayamaOsaka Metropolitan University YUYuhichi UedaMaizuru Municipal Hospital HKHiroyoshi KawamotoGunma Prefectural Institute of Public Health and Environmental Sciences

Discussion

Member takes

Implication

Norwalk-like viruses contribute to sporadic and outbreak gastroenteritis in Japan; leaves open prospective validation for routine RT-PCR diagnostics.

Study Design

Type

Cross-Sectional (n=395)

Multicenter

Yes

Structured PICO

Population

395 stool samples from patients with gastroenteritis in Japan, including sporadic cases and outbreaks, analyzed for Norwalk-like viruses.

Exposure

Reverse transcription-polymerase chain reaction (RT-PCR) amplification and DNA sequencing

Outcome

Detection rate and DNA sequence of Norwalk-like virusessurrogate

RT-PCR successfully detected Norwalk-like viruses in sporadic and outbreak gastroenteritis cases in Japan, identifying two main subgenogroups.

Cite This Study

Nakayama et al. (1996) conducted a cross-sectional in Gastroenteritis (n=395). Reverse transcription-polymerase chain reaction (RT-PCR) was evaluated on Detection of Norwalk-like viruses. Norwalk-like viruses were detected by RT-PCR in 7% of all sporadic gastroenteritis cases and 15 of 17 outbreak cases in Japan, with two subgenogroups identified.

synapsesocial.com/papers/6a1fc8052be0c1f4e3bb417b https://doi.org/10.1111/j.1348-0421.1996.tb03343.x

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Implication

Norwalk-like viruses contribute to sporadic and outbreak gastroenteritis in Japan; leaves open prospective validation for routine RT-PCR diagnostics.

Detection and Sequencing of Norwalk‐Like Viruses from Stool Samples in Japan Using Reverse Transcription‐Polymerase Chain Reaction Amplification

Discussion

Member takes

Implication

Study Design

Structured PICO

Cite This Study

Detection and Sequencing of Norwalk‐Like Viruses from Stool Samples in Japan Using Reverse Transcription‐Polymerase Chain Reaction Amplification

Discussion

Member takes

Implication

Study Design

Structured PICO

Cite This Study

Also Consider

Also Consider