January 1, 1989Nucleic Acids ResearchOpen Access

Design of RNA enzymes distinguishing a single base mutation in RNA

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Population

In vitro RNA substrates including synthetic DNA duplexes coding for positions 7-26 of c-Ha-ras protein

Comparison

RNA enzymes designed to recognize sequences of… vs Wild type mRNA (GGU)

Design

Preclinical

Authors

Discussion

No immediate clinical application; leaves open further development of allele-specific ribozymes.

Population

In vitro RNA substrates including synthetic DNA duplexes coding for positions 7-26 of c-Ha-ras protein (mutant Val-12 mRNA and wild type mRNA)

Intervention

RNA enzymes (ribozymes) designed to recognize sequences of 9-15 bases

Comparator

Wild type mRNA (GGU)

Outcome

RNA cleavagesurrogate

Designed ribozymes can successfully distinguish and selectively cleave RNA with a single base mutation in vitro.

Koizumi et al. (1989) studied this question.

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