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April 1, 1990Proceedings of the National Academy of SciencesOpen Access

Molecular epidemiology of human hepatitis A virus defined by an antigen-capture polymerase chain reaction method.

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Why the study?

Does the antigen-capture/polymerase chain reaction (AC/PCR) method enable the detection and molecular epidemiological study of hepatitis A virus?

Population

34 virus strains of hepatitis A virus (HAV) recovered from clinical specimens

Design

Preclinical

Authors

RJRobert W. JansenUniversity of North Carolina at Chapel HillGSG. SieglUniversity of North Carolina at Chapel HillSLStanley M. LemonUniversity of North Carolina at Chapel Hill

Discussion

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Implication

AC/PCR may advance preclinical HAV research; leaves open clinical validation and human outbreak applications.

Key Points

  • The study aims to develop a method for detecting hepatitis A virus using antigen-capture PCR.
  • Developed an immunoaffinity-linked nucleic acid amplification system (AC/PCR).
  • Conducted sequencing of AC/PCR reaction products from 34 virus strains.
  • Analyzed viral cDNA amplification in a single reaction vessel.
  • AC/PCR detected less than one cell culture infectious unit of virus in 80 microliters of sample.
  • Most virus strains showed remarkable conservation at the nucleotide level, with unexpected genetic diversity among human isolates.
  • Identical sequences from viruses in outbreaks in the United States and Germany indicate a previously unrecognized epidemiologic link.

Structured PICO

Does the antigen-capture/polymerase chain reaction (AC/PCR) method enable the detection and molecular epidemiological study of hepatitis A virus?

P
Population
34 virus strains of hepatitis A virus (HAV) recovered from clinical specimens
I
Intervention
Antigen-capture/polymerase chain reaction (AC/PCR) method
O
Outcome
Detection of virus and sequencing of AC/PCR reaction productssurrogate

The AC/PCR method simplifies sample preparation, enhances specificity, and enables molecular epidemiological tracking of hepatitis A virus outbreaks.

Cite This Study

Jansen et al. (1990) studied this question.

synapsesocial.com/papers/6a1fe831702b8f8c062e4dc8https://doi.org/10.1073/pnas.87.8.2867
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Characterization of a simian hepatitis A virus (HAV): antigenic and genetic comparison with human HAV1989 · 83 citations
  2. 2Molecular Cloning of cDNA from Hepatitis A Virus Strain HM-175 after Multiple Passages in vivo and in vitro1986 · 13 citations
  3. 3Molecular cloning and partial sequencing of hepatitis A viral cDNA1985 · 75 citations
  4. 4Primary structure and gene organization of human hepatitis A virus.1985 · 200 citations
  5. 5Propagation of Human Hepatitis A Virus in Cell Culture in Vitro1979 · 392 citations