Key result
Specific antipeptide antibodies to the external vestibules of Kv1.2 and Kv3.1 reduced whole-cell potassium currents in transfected cells, an effect blocked by the corresponding peptide antigen.
Why the study?
Do specific antipeptide antibodies to the external vestibule of voltage-gated potassium channels block whole-cell currents in transfected and neuronal cells?
Population
Transfected cells expressing Kv1.2 or Kv3.1 channel proteins, and NG108 neuronal cells
Comparison
Specific antipeptide antibodies to epitopes in… vs Control peptides and control antibodies
Design
Preclinical
Authors
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Succeeds in blocking Kv currents via vestibule antibodies in vitro; extends basic tools but leaves open cardiovascular applications.
Do specific antipeptide antibodies to the external vestibule of voltage-gated potassium channels block whole-cell currents in transfected and neuronal cells?
The design of specific antipeptide antibodies targeting the external vestibule of voltage-gated potassium channels successfully blocks their currents, providing a novel tool for physiological studies of ion channel functions.
Zhou et al. (1998) studied this question. Specific antipeptide antibodies to epitopes in the external vestibules of Kv1.2 and Kv3.1 vs. Control antibodies and control peptides was evaluated on Reduction in whole-cell Kv1.2 or Kv3.1 currents. Specific antipeptide antibodies to the external vestibules of Kv1.2 and Kv3.1 reduced whole-cell potassium currents in transfected cells, an effect blocked by the corresponding peptide antigen.
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