What question did this study set out to answer?

To determine whether human pulmonary cells synthesize and secrete extracellular glutathione peroxidase into epithelial lining fluid to defend the respiratory tract against oxidant injury.

February 1, 1996AJP Lung Cellular and Molecular Physiology

Extracellular glutathione peroxidase in human lung epithelial lining fluid and in lung cells

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Authors

NANelly E. AvissarTel Aviv University JFJ. N. FinkelsteinInstitut Sophia Agrobiotech SHStuart HorowitzNorthwestern University

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Implication

Experimental study reveals extracellular glutathione peroxidase drives most peroxidase activity in lung fluid, indicating airway cells supply protective antioxidants.

Key Points

To determine whether human pulmonary cells synthesize and secrete extracellular glutathione peroxidase into epithelial lining fluid to defend the respiratory tract against oxidant injury.
Quantified extracellular versus cellular glutathione peroxidase activity in human epithelial lining fluid using specific immunoprecipitation assays.
Cultured primary human bronchial epithelial cells, alveolar macrophages, and cell lines (BEP2D, A549) with 75Se metabolic labeling to detect enzyme secretion.
Analyzed gene expression in human and animal lung cells and tissue sections using Northern blot, RT-PCR, and in situ hybridization.
Extracellular glutathione peroxidase accounted for 57% of total glutathione peroxidase activity in epithelial lining fluid, while cellular glutathione peroxidase accounted for 40%.
Primary bronchial epithelial cells, alveolar macrophages, BEP2D, and A549 synthesized both enzymes and secreted extracellular glutathione peroxidase into culture media.
Freshly isolated human bronchial epithelial cells and alveolar macrophages expressed eGPx mRNA, which localized primarily within interstitial cells surrounding airway walls.

Cite This Study

Avissar et al. (1996) studied this question.

synapsesocial.com/papers/6a1ff5a691899344c12e652e https://doi.org/10.1152/ajplung.1996.270.2.l173

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Implication

Experimental study reveals extracellular glutathione peroxidase drives most peroxidase activity in lung fluid, indicating airway cells supply protective antioxidants.

Extracellular glutathione peroxidase in human lung epithelial lining fluid and in lung cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Extracellular glutathione peroxidase in human lung epithelial lining fluid and in lung cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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