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Expression and functionality of P-glycoprotein in human bronchial epithelial cells in vitro

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Researchers evaluate P-glycoprotein functionality in bronchial epithelial cells, suggesting complex transport roles.

The research aims to explore the expression and functionality of P-glycoprotein in human bronchial epithelial cells.
Cultured Calu-3 and NHBE cells at an air-liquid interface for 21 days
Evaluated P-gp expression using quantitative polymerase chain reaction
Assessed functionality through permeability measurements with 3H-digoxin and inhibitors
P-glycoprotein was absent in NHBE cells and moderately expressed in Calu-3 cells
Net secretory transport of 3H-digoxin was observed in both models
P-glycoprotein activity was reduced by temperature reduction and specific inhibitors
No effect on transport was noted with metabolic inhibitors in Calu-3 cells

Hutter et al. (2011) studied this question.

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