What question did this study set out to answer?

To determine whether cyclin levels directly regulate G1 phase progression and proliferation commitment in mammalian cells.

March 26, 1993Science

Cyclin-Dependent Regulation of G 1 in Mammalian Fibroblasts

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Authors

MOMotoaki OhtsuboKurume University JRJames M. RobertsUniversity of California, Davis

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Implication

Experimental study demonstrates that cyclin E overexpression accelerates G1 phase progression in mammalian fibroblasts, indicating cyclin levels are rate-limiting for cell proliferation.

Key Points

To determine whether cyclin levels directly regulate G1 phase progression and proliferation commitment in mammalian cells.
Generated mammalian fibroblast cell lines engineered to constitutively overexpress human cyclin E.
Evaluated G1 phase duration, cell size alterations, and serum requirements during the transition from G1 to S phase.
Constitutive overexpression of human cyclin E shortened the duration of the G1 phase and reduced cell size.
Cyclin E overexpression decreased the serum threshold required for cells to advance from G1 to S phase.
Cyclin abundance functions as a rate-limiting factor governing G1 progression and cell cycle commitment in mammalian cells.

Cite This Study

Ohtsubo et al. (1993) studied this question.

synapsesocial.com/papers/6a1ffb76d4e6d3589704c2e8 https://doi.org/10.1126/science.8384376

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March 26, 1993Science

Cyclin-Dependent Regulation of G 1 in Mammalian Fibroblasts

View Full Paper

Ask AI

Bookmark

Authors

MOMotoaki OhtsuboKurume University JRJames M. RobertsUniversity of California, Davis

Discussion

Member takes

Implication

Experimental study demonstrates that cyclin E overexpression accelerates G1 phase progression in mammalian fibroblasts, indicating cyclin levels are rate-limiting for cell proliferation.

Key Points

To determine whether cyclin levels directly regulate G1 phase progression and proliferation commitment in mammalian cells.
Generated mammalian fibroblast cell lines engineered to constitutively overexpress human cyclin E.
Evaluated G1 phase duration, cell size alterations, and serum requirements during the transition from G1 to S phase.
Constitutive overexpression of human cyclin E shortened the duration of the G1 phase and reduced cell size.
Cyclin E overexpression decreased the serum threshold required for cells to advance from G1 to S phase.
Cyclin abundance functions as a rate-limiting factor governing G1 progression and cell cycle commitment in mammalian cells.

Cite This Study

Ohtsubo et al. (1993) studied this question.

synapsesocial.com/papers/6a1ffb76d4e6d3589704c2e8 https://doi.org/10.1126/science.8384376

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